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Image Search Results


Histopathological profiles in the groups

Journal: Vascular medicine (London, England)

Article Title: Incremental Effects of Diabetes Mellitus and Chronic Kidney Disease in Medial Arterial Calcification: Synergistic Pathways for Peripheral Artery Disease Progression

doi: 10.1177/1358863X19842276

Figure Lengend Snippet: Histopathological profiles in the groups

Article Snippet: Primary antibodies against the following antigens were used: rabbit polyclonal a-SMA (PA1–37024-Thermofisher, IL, 1:100 dilution), mouse monoclonal a-SMA (FITC-F3777-Sigma Aldrich, MO, 1:500 dilution), mouse monoclonal BMP2 (ab6285-Abcam, MA, 1:100 dilution), and Fetuin-A (NBP1–91192 -Novus Biologicals, CO, 1:200 dilution).

Techniques: Staining, Expressing

BMP-2 density (A-D): Immunostaining for BMP-2 using diaminobenzene brown chromogen labeling, showing BMP-2 expression in the intimal and medial layers of peripheral atherosclerotic plaques from patients without DM or CKD (A), DM only (B), CKD only (C) and DM+CKD (D) (20 X magnification, scale bar=50μm). E) Scatter gram shows results of quantitative analysis of BMP-2 protein density levels in peripheral plaques among groups, No-DM/no-CKD, (n=5); DM(n=6); CKD (n=6); DM+CKD (n=8): (n = observations/high power field); Univariate Analysis of Variance with Bonferroni’s post-hoc test. *** p<0.001 vs. no-DM/no-CKD. F-I)

Journal: Vascular medicine (London, England)

Article Title: Incremental Effects of Diabetes Mellitus and Chronic Kidney Disease in Medial Arterial Calcification: Synergistic Pathways for Peripheral Artery Disease Progression

doi: 10.1177/1358863X19842276

Figure Lengend Snippet: BMP-2 density (A-D): Immunostaining for BMP-2 using diaminobenzene brown chromogen labeling, showing BMP-2 expression in the intimal and medial layers of peripheral atherosclerotic plaques from patients without DM or CKD (A), DM only (B), CKD only (C) and DM+CKD (D) (20 X magnification, scale bar=50μm). E) Scatter gram shows results of quantitative analysis of BMP-2 protein density levels in peripheral plaques among groups, No-DM/no-CKD, (n=5); DM(n=6); CKD (n=6); DM+CKD (n=8): (n = observations/high power field); Univariate Analysis of Variance with Bonferroni’s post-hoc test. *** p<0.001 vs. no-DM/no-CKD. F-I)

Article Snippet: Primary antibodies against the following antigens were used: rabbit polyclonal a-SMA (PA1–37024-Thermofisher, IL, 1:100 dilution), mouse monoclonal a-SMA (FITC-F3777-Sigma Aldrich, MO, 1:500 dilution), mouse monoclonal BMP2 (ab6285-Abcam, MA, 1:100 dilution), and Fetuin-A (NBP1–91192 -Novus Biologicals, CO, 1:200 dilution).

Techniques: Immunostaining, Labeling, Expressing

SMCs Expressing BMP2 Immunofluorescence staining for α-SMA (red) and BMP2 (green) in peripheral atherosclerotic plaques with MAC from patients without DM or CKD (A), DM only (B), CKD only (C) and DM+CKD (D); SMCs positive for BMP2 visualized in yellow (20 X magnification, scale bar=20μm). E) Scatter gram shows results of quantitative analysis of SMCs (α-SMA+) expressing BMP2 in peripheral atherosclerotic plaques among groups; No-DM/no-CKD, (n=8); DM(n=13); CKD (n=9); DM+CKD (n=16): (n = observations /high power field); Univariate Analysis of Variance with Bonferroni’s post-hoc test. * p<0.05, *** p<0.001 vs. no-DM/no-CKD.

Journal: Vascular medicine (London, England)

Article Title: Incremental Effects of Diabetes Mellitus and Chronic Kidney Disease in Medial Arterial Calcification: Synergistic Pathways for Peripheral Artery Disease Progression

doi: 10.1177/1358863X19842276

Figure Lengend Snippet: SMCs Expressing BMP2 Immunofluorescence staining for α-SMA (red) and BMP2 (green) in peripheral atherosclerotic plaques with MAC from patients without DM or CKD (A), DM only (B), CKD only (C) and DM+CKD (D); SMCs positive for BMP2 visualized in yellow (20 X magnification, scale bar=20μm). E) Scatter gram shows results of quantitative analysis of SMCs (α-SMA+) expressing BMP2 in peripheral atherosclerotic plaques among groups; No-DM/no-CKD, (n=8); DM(n=13); CKD (n=9); DM+CKD (n=16): (n = observations /high power field); Univariate Analysis of Variance with Bonferroni’s post-hoc test. * p<0.05, *** p<0.001 vs. no-DM/no-CKD.

Article Snippet: Primary antibodies against the following antigens were used: rabbit polyclonal a-SMA (PA1–37024-Thermofisher, IL, 1:100 dilution), mouse monoclonal a-SMA (FITC-F3777-Sigma Aldrich, MO, 1:500 dilution), mouse monoclonal BMP2 (ab6285-Abcam, MA, 1:100 dilution), and Fetuin-A (NBP1–91192 -Novus Biologicals, CO, 1:200 dilution).

Techniques: Expressing, Immunofluorescence, Staining